ALIX-His(1-868, P801G)
(Plasmid
#186808)
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PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186808 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-11a
- Backbone size w/o insert (bp) 5641
- Total vector size (bp) 8266
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameALIX-His (1-868, P801G)
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SpeciesH. sapiens (human)
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Insert Size (bp)2625
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MutationProline 801 to Glycine
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Entrez GenePDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
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Tag
/ Fusion Protein
- His6 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ALIX UniProt ID: Q8WUM4
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ALIX-His(1-868, P801G) was a gift from Lalit Deshmukh (Addgene plasmid # 186808 ; http://n2t.net/addgene:186808 ; RRID:Addgene_186808) -
For your References section:
Avidity-Based Method for the Efficient Generation of Monoubiquitinated Recombinant Proteins. Nelson SL, Li Y, Chen Y, Deshmukh L. J Am Chem Soc. 2023 Apr 3. doi: 10.1021/jacs.3c01943. 10.1021/jacs.3c01943 PubMed 37010382
