His-Tev-UbE2D3
(Plasmid #186805)

• Purpose: Codon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.
• Depositing Lab: Lalit Deshmukh
• Publication: Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/jacs.3c01943.

Backbone

• Vector backbone: pET-11a
• Backbone size w/o insert (bp): 5632
• Total vector size (bp): 6136
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: His-Tev-UbE2D3
• Alt name: UBCH5C
• Species: H. sapiens (human)
• Insert Size (bp): 504
• Entrez Gene: UBE2D3 (a.k.a. E2(17)KB3, UBC4/5, UBCH5C)
• Tag / Fusion Protein:
  • His6-Tev (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

UbE2D3 UniProt ID: P61077