His-Tev-UbE1
(Plasmid #186804)

• Purpose: Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.
• Depositing Lab: Lalit Deshmukh
• Publication: Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/jacs.3c01943.

Backbone

• Vector backbone: pET-11a
• Backbone size w/o insert (bp): 5632
• Total vector size (bp): 8860
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: His-Tev-UbE1
• Species: H. sapiens (human)
• Insert Size (bp): 3227
• Entrez Gene: UBA1 (a.k.a. A1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, VEXAS)
• Tag / Fusion Protein:
  • His6-Tev (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

UbE1 UniProt ID: P22314