Twin-Strep(R)-Tev-GSGS-Ubiquitin
(Plasmid #186803)

• Purpose: Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.
• Depositing Lab: Lalit Deshmukh
• Publication: Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/jacs.3c01943.

Backbone

• Vector backbone: pET-11a
• Backbone size w/o insert (bp): 5632
• Total vector size (bp): 6004
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Twin-Strep(R)-Tev-GSGS-Ubiquitin
• Alt name: Ubiquitin
• Species: H. sapiens (human)
• Insert Size (bp): 372
• Mutation: Strep tag Lysine's mutated to Arginine
• Tag / Fusion Protein:
  • GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER)-TEV-GSGS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ubiquitin UniProt ID: P0CG48