cytoplasmic EKAR (Cerulean-Venus)
(Plasmid #18679)

• Depositing Lab: Karel Svoboda
• Publication: Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ERK Activity Reporter
• Alt name: EKAR
• Insert Size (bp): 550
• Tags / Fusion Proteins:
  • monomeric Cerulean (N terminal on insert)
  • monomeric Venus (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: David Yue (Glycine linker) David Litchfield (Pin1) Deborah Morrison (Cdc25C)
• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry