Skip to main content
Addgene

PB533-42B3mutAC2-sfGFP
(Plasmid #186777)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186777 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    PB533
  • Total vector size (bp) 8351
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    42B3mutAC2-scFv
  • Alt name
    42B3(R78K/A80T/M95V)-scFv
  • Insert Size (bp)
    734
  • GenBank ID
    LC628457
  • Tag / Fusion Protein
    • sfGFP

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB533-42B3mutAC2-sfGFP was a gift from Hiroshi Kimura (Addgene plasmid # 186777 ; http://n2t.net/addgene:186777 ; RRID:Addgene_186777)

  • For your References section:

    Live imaging of transcription sites using an elongating RNA polymerase II-specific probe. Uchino S, Ito Y, Sato Y, Handa T, Ohkawa Y, Tokunaga M, Kimura H. J Cell Biol. 2022 Feb 7;221(2). pii: 212888. doi: 10.1083/jcb.202104134. Epub 2021 Dec 2. 10.1083/jcb.202104134 PubMed 34854870
Related items:
Commonly requested with: