pU1_000
(Plasmid
#186773)
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Purpose(Empty Backbone) * Engineered U1 snRNA plasmid for insertion of guide sequences.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186773 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
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Backbone manufacturerNEB
- Backbone size (bp) 2500
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Vector typeMammalian Expression, Bacterial Expression, CRISPR
- Promoter U1 Promoter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pU1_000 was a gift from Eugene Yeo (Addgene plasmid # 186773 ; http://n2t.net/addgene:186773 ; RRID:Addgene_186773) -
For your References section:
Engineered U1 snRNAs to modulate alternatively spliced exons. Hatch ST, Smargon AA, Yeo GW. Methods. 2022 Jun 25;205:140-148. doi: 10.1016/j.ymeth.2022.06.008. 10.1016/j.ymeth.2022.06.008 PubMed 35764245
Map uploaded by the depositor.
