pDONR/Zeo-sfGFP-SKL
(Plasmid
#186720)
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PurposeGateway entry vector encoding sfGFP with the two peroxisome-targeting signals (out of frame).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR/Zeo
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namesfGFP-SKL
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Alt namesfGFP-the peroxisome-targeting signal
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR/Zeo-sfGFP-SKL was a gift from Yutaka Kodama (Addgene plasmid # 186720 ; http://n2t.net/addgene:186720 ; RRID:Addgene_186720) -
For your References section:
SKLPT imaging: Efficient in vivo pre-evaluation of genome-editing modules using fluorescent protein with peroxisome targeting signal. Konno R, Tanaka H, Kodama Y. Biochem Biophys Res Commun. 2018 Sep 3;503(1):235-241. doi: 10.1016/j.bbrc.2018.06.008. Epub 2018 Jun 12. 10.1016/j.bbrc.2018.06.008 PubMed 29885839