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Addgene

hCtRNA_FT
(Plasmid #186715)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186715 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Custom
  • Backbone size w/o insert (bp) 2216
  • Total vector size (bp) 2367
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA scaffold and hCtRNA
  • Alt name
    GP3.4
  • Species
    Synthetic
  • Insert Size (bp)
    151
  • Promoter hCtRNA

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CAAATAGGGGTTCCGCGCACAT
  • 3′ sequencing primer GAAGGGAGAAAGGCGGACAGGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCtRNA_FT was a gift from Xue Gao (Addgene plasmid # 186715 ; http://n2t.net/addgene:186715 ; RRID:Addgene_186715)

  • For your References section:

    Multiplex base- and prime-editing with drive-and-process CRISPR arrays. Yuan Q, Gao X. Nat Commun. 2022 May 19;13(1):2771. doi: 10.1038/s41467-022-30514-1. 10.1038/s41467-022-30514-1 PubMed 35589728
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