pSGNluc
(Plasmid
#186705)
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PurposeA high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameGFP
Gene/Insert 2
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Gene/Insert nameluciferase
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSGNluc was a gift from Baubak Bajoghli & Maria Leptin (Addgene plasmid # 186705 ; http://n2t.net/addgene:186705 ; RRID:Addgene_186705) -
For your References section:
A high-sensitivity bi-directional reporter to monitor NF-kappaB activity in cell culture and zebrafish in real time. Kuri P, Ellwanger K, Kufer TA, Leptin M, Bajoghli B. J Cell Sci. 2017 Feb 1;130(3):648-657. doi: 10.1242/jcs.196485. Epub 2016 Dec 15. 10.1242/jcs.196485 PubMed 27980067