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pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH)
(Plasmid #186703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186703 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPBbleo
  • Backbone size w/o insert (bp) 6700
  • Total vector size (bp) 10052
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    miRFP703-eDHFR(69K6)-Tiam1(DH-PH)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3318
  • GenBank ID

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Shinya Tsukiji (Addgene plasmid # 172102: pPBpuro-mNG-eDHFR(69K6)-Tiam1)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The chemical mDcTMP, to control protein translocation, can be obtained from Funakoshi Co., Ltd. https://www.funakoshi.co.jp/exports_contents/95015

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBbleo-miRFP703-eDHFR(69K6)-Tiam1(DH-PH) was a gift from Michiyuki Matsuda (Addgene plasmid # 186703 ; http://n2t.net/addgene:186703 ; RRID:Addgene_186703)

  • For your References section:

    A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. Hino N, Matsuda K, Jikko Y, Maryu G, Sakai K, Imamura R, Tsukiji S, Aoki K, Terai K, Hirashima T, Trepat X, Matsuda M. Dev Cell. 2022 Oct 10;57(19):2290-2304.e7. doi: 10.1016/j.devcel.2022.09.003. Epub 2022 Sep 28. 10.1016/j.devcel.2022.09.003 PubMed 36174555
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