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Addgene

pBFC0996
(Plasmid #186695)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186695 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    R6K conditional origin, bla, RP4 oriT
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EC100D pir+
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    tnsA
  • Species
    Vibrio cholerae
  • Promoter PLac

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    tnsB
  • Species
    Vibrio cholerae

Gene/Insert 3

  • Gene/Insert name
    tnsC
  • Species
    Vibrio cholerae

Gene/Insert 4

  • Gene/Insert name
    tnsD
  • Species
    Vibrio cholerae

Gene/Insert 5

  • Gene/Insert name
    tniQ
  • Species
    Vibrio cholerae

Gene/Insert 6

  • Gene/Insert name
    cas8-cas5 fusion
  • Species
    Vibrio cholerae

Gene/Insert 7

  • Gene/Insert name
    cas7
  • Species
    Vibrio cholerae

Gene/Insert 8

  • Gene/Insert name
    cas6
  • Species
    Vibrio cholerae

Gene/Insert 9

  • Gene/Insert name
    crRNA
  • Species
    Vibrio cholerae

Gene/Insert 10

  • Gene/Insert name
    Tn7 transposon
  • Species
    Vibrio cholerae

Gene/Insert 11

  • Gene/Insert name
    2xLguI Cargo Stuffer

Gene/Insert 12

  • Gene/Insert name
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Clone guides using the 2xBsaI cloning site.
Clone Tn barcodes using the 2xBsmBI cloning site.
Clone any VcDART cargo of interest using the 2xLguI cargo cloning site inside the Tn7 transposon.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBFC0996 was a gift from Jennifer Doudna (Addgene plasmid # 186695 ; http://n2t.net/addgene:186695 ; RRID:Addgene_186695)

  • For your References section:

    Species- and site-specific genome editing in complex bacterial communities. Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL, Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM, Banfield JF, Doudna JA. Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7. Epub 2021 Dec 6. 10.1038/s41564-021-01014-7 PubMed 34873292
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