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pBFC0687
(Plasmid #186692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    R6K conditional origin, bla, RP4 oriT
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Gentamicin, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EC100D pir+
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    tnsB
  • Species
    Scytonema hofmanni
  • Promoter PLac

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    tnsC
  • Species
    Scytonema hofmanni

Gene/Insert 3

  • Gene/Insert name
    tniQ
  • Species
    Scytonema hofmanni

Gene/Insert 4

  • Gene/Insert name
    Cas12k
  • Alt name
    ShCas12k
  • Species
    Scytonema hofmanni

Gene/Insert 5

  • Gene/Insert name
    Sh_2xBsaI_NT (Guide Stuffer) gRNA
  • Species
    Scytonema hofmanni

Gene/Insert 6

  • Gene/Insert name
    Tn7 transposon
  • Species
    Scytonema hofmanni

Gene/Insert 7

  • Gene/Insert name
    GmR+sfGFP ShDART Cargo
  • Alt name
    gentmycin marker+sfGFP ShDART Cargo

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Feng Zhang lab's ShCAST plasmids: Addgene #127921 and #127924

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Clone guides using the 2xBsaI cloning site.
Clone Tn barcodes using the 2xBsmBI cloning site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBFC0687 was a gift from Jennifer Doudna (Addgene plasmid # 186692 ; http://n2t.net/addgene:186692 ; RRID:Addgene_186692)

  • For your References section:

    Species- and site-specific genome editing in complex bacterial communities. Rubin BE, Diamond S, Cress BF, Crits-Christoph A, Lou YC, Borges AL, Shivram H, He C, Xu M, Zhou Z, Smith SJ, Rovinsky R, Smock DCJ, Tang K, Owens TK, Krishnappa N, Sachdeva R, Barrangou R, Deutschbauer AM, Banfield JF, Doudna JA. Nat Microbiol. 2022 Jan;7(1):34-47. doi: 10.1038/s41564-021-01014-7. Epub 2021 Dec 6. 10.1038/s41564-021-01014-7 PubMed 34873292
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