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Addgene

VAMP8
(Plasmid #186689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTEV5
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VAMP8
  • Species
    R. norvegicus (rat)
  • Promoter T7
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • Thrombin
    • TEV
    • 3xFLAG

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VAMP8 was a gift from Axel Brunger (Addgene plasmid # 186689 ; http://n2t.net/addgene:186689 ; RRID:Addgene_186689)

  • For your References section:

    Inhibition of calcium-triggered secretion by hydrocarbon-stapled peptides. Lai Y, Fois G, Flores JR, Tuvim MJ, Zhou Q, Yang K, Leitz J, Peters J, Zhang Y, Pfuetzner RA, Esquivies L, Jones P, Frick M, Dickey BF, Brunger AT. Nature. 2022 Mar;603(7903):949-956. doi: 10.1038/s41586-022-04543-1. Epub 2022 Mar 23. 10.1038/s41586-022-04543-1 PubMed 35322233
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