Syt2
(Plasmid
#186688)
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PurposeExpresses Synaptotagmin 2 in E. Coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 186688 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTEV5
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSynaptotagmin 2
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SpeciesR. norvegicus (rat)
- Promoter T7
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Tags
/ Fusion Proteins
- GST (N terminal on insert)
- thrombin site (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Syt2 was a gift from Axel Brunger (Addgene plasmid # 186688 ; http://n2t.net/addgene:186688 ; RRID:Addgene_186688) -
For your References section:
Inhibition of calcium-triggered secretion by hydrocarbon-stapled peptides. Lai Y, Fois G, Flores JR, Tuvim MJ, Zhou Q, Yang K, Leitz J, Peters J, Zhang Y, Pfuetzner RA, Esquivies L, Jones P, Frick M, Dickey BF, Brunger AT. Nature. 2022 Mar;603(7903):949-956. doi: 10.1038/s41586-022-04543-1. Epub 2022 Mar 23. 10.1038/s41586-022-04543-1 PubMed 35322233