MUNC18-2
(Plasmid
#186687)
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PurposeExpresses MUNC18-2 in insect cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 186687 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFASTBac
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Vector typeInsect Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MUNC18-2 was a gift from Axel Brunger (Addgene plasmid # 186687 ; http://n2t.net/addgene:186687 ; RRID:Addgene_186687) -
For your References section:
Inhibition of calcium-triggered secretion by hydrocarbon-stapled peptides. Lai Y, Fois G, Flores JR, Tuvim MJ, Zhou Q, Yang K, Leitz J, Peters J, Zhang Y, Pfuetzner RA, Esquivies L, Jones P, Frick M, Dickey BF, Brunger AT. Nature. 2022 Mar;603(7903):949-956. doi: 10.1038/s41586-022-04543-1. Epub 2022 Mar 23. 10.1038/s41586-022-04543-1 PubMed 35322233