pBZ-attBtxnl4b
(Plasmid #18667)

• Purpose: cloning vector containing attB flanked TXNL4B useful for recombination based conversion of conventional vectors to Gateway compatible destination vectors
• Depositing Lab: Yu-Zhu Zhang
• Publication: Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Backbone

• Vector backbone: pBZ
• Backbone size w/o insert (bp): 2930
• Total vector size (bp): 3495
• Modifications to backbone: pBZ is a derivative of pBlueScript II SK with a modified multiple cloning site (MCS).
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: thioredoxin-like 4B
• Alt name: TXNL4B
• Alt name: human Dim2
• Species: H. sapiens (human)
• Insert Size (bp): 565
• Entrez Gene: TXNL4B (a.k.a. DLP, Dim2, FLJ20511)
• Promoter: T3

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

attB cassette flanking coding region of TXNL4B (dim2) can be released by digestion with NotI and BamHI