pJMH147
(Plasmid
#186647)
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PurposeaadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1 between homology arms for pilA
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186647 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJMH020
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Backbone manufacturerGenscript
- Backbone size w/o insert (bp) 4636
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Vector typeBacterial Expression, Synthetic Biology
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Selectable markersStreptomycin/Spectinomycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameaadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1
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Insert Size (bp)3959
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGenscript Corp.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.06.30.498297v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJMH147 was a gift from Adam Arkin (Addgene plasmid # 186647 ; http://n2t.net/addgene:186647 ; RRID:Addgene_186647) -
For your References section:
Acetaminophen production in the edible, filamentous cyanobacterium Arthrospira platensis. Jacob M. Hilzinger, Skyler Friedline, Divya Sivanandan, Ya-Fang Cheng, Shunsuke Yamazaki, Douglas S. Clark, Jeffrey M. Skerker, Adam P. Arkin. bioRxiv 10.1101/2022.06.30.498297