mRFP-RBD(R59A)-mRFP
(Plasmid #18664)

• Depositing Lab: Karel Svoboda
• Publication: Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Backbone

• Vector backbone: pCI
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ras binding domain of Raf
• Alt name: RBD
• Insert Size (bp): 250
• Mutation: R59A mutation in RBD
• Tags / Fusion Proteins:
  • mRFP (N terminal on insert)
  • mRFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV Forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: M. Matsuda (Osaka University)
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry