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Addgene

pTE1069
(Plasmid #186629)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186629 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    BC-A1-002 (Addgene #78689)
  • Backbone size w/o insert (bp) 2200
  • Total vector size (bp) 3987
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    scbR
  • Alt name
    SCO6265
  • Species
    Streptomyces coelicolor
  • Insert Size (bp)
    645
  • Promoter BBa_J23100
  • Tag / Fusion Protein
    • 6xArg (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GTTGACGGCTAGCTCAGTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    scbAp promoter
  • Species
    Streptomyces coelicolor
  • Insert Size (bp)
    35
  • Promoter scbAp

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTE1069 was a gift from Eriko Takano (Addgene plasmid # 186629 ; http://n2t.net/addgene:186629 ; RRID:Addgene_186629)

  • For your References section:

    Orthogonal Regulatory Circuits for Escherichia coli Based on the gamma-Butyrolactone System of Streptomyces coelicolor. Biarnes-Carrera M, Lee CK, Nihira T, Breitling R, Takano E. ACS Synth Biol. 2018 Apr 20;7(4):1043-1055. doi: 10.1021/acssynbio.7b00425. Epub 2018 Mar 19. 10.1021/acssynbio.7b00425 PubMed 29510026
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