pLV-mCherry-Stim1
(Plasmid #186620)

• Purpose: Third generation lentiviral vector expressing Stim1-wt fused to mCherry
• Depositing Lab: Gia Voeltz
• Publication: Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6. doi: 10.1016/j.devcel.2022.05.002. Epub 2022 May 23.

Backbone

• Vector backbone: pLV-eGFP_Addgene#36083
• Total vector size (bp): 9462
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Stromal Interaction Molecule 1
• Alt name: Stim1
• Species: H. sapiens (human)
• Insert Size (bp): 2055
• Entrez Gene: STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: aggtctatataagcagagctcgtttag
• 3′ sequencing primer: aaagcagcgtatccacatagc

Resource Information

• Supplemental Documents:
  • pLV-mCherry-Stim1-wt.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry