mEGFP-HRas
(Plasmid #18662)

• Depositing Lab: Karel Svoboda
• Publication: Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Backbone

• Vector backbone: pCI
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H-Ras
• Alt name: HRas
• Species: H. sapiens (human)
• Insert Size (bp): 600
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
• Promoter: CMV
• Tag / Fusion Protein:
  • monomeric EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: EGFP-C; chim-int-F (5'-TCTTACTGACATCCACTTTGCC-3')
• 3′ sequencing primer: EBV-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: M. Matsuda (Osaka University)
• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry