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Addgene

pLitTREPitt
(Plasmid #18661)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18661 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLitmus 29
  • Backbone size w/o insert (bp) 2812

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TREPitt
  • Species
    Synthetic DNA
  • Insert Size (bp)
    409

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI, HindIII, NcoI (unknown if destroyed)
  • 3′ cloning site AgeI, AvrII, SpeI, SnaBI (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLitTREPitt was a gift from Jakob Reiser (Addgene plasmid # 18661 ; http://n2t.net/addgene:18661 ; RRID:Addgene_18661)

  • For your References section:

    Tight control of transgene expression by lentivirus vectors containing second-generation tetracycline-responsive promoters. Pluta K, Luce MJ, Bao L, Agha-Mohammadi S, Reiser J. J Gene Med. 2005 Jun . 7(6):803-17. 10.1002/jgm.712 PubMed 15655804
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