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Rtn2b-RHD272-472-mNeon
(Plasmid #186605)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186605 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mNeonGreen-N1
  • Total vector size (bp) 5314
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Human Reticulon 2 isoform B amino acids 272 to 472
  • Alt name
    Rtn2b-RHD272-472
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    603
  • Mutation
    Met-Gly-Ser-Lys peptide added at N-terminus for correct insertion at ER membrane
  • GenBank ID
    NM_206900.3
  • Entrez Gene
    RTN2 (a.k.a. NSP2, NSPL1, NSPLI, SPG12)
  • Promoter CMV
  • Tag / Fusion Protein
    • mNeonGreen (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer tgtaactcatgtgtcgctggg
  • 3′ sequencing primer gacgcaaatgggcggtagg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rtn2b-RHD272-472-mNeon was a gift from Gia Voeltz (Addgene plasmid # 186605 ; http://n2t.net/addgene:186605 ; RRID:Addgene_186605)

  • For your References section:

    The ER ladder is a unique morphological feature of developing mammalian axons. Zamponi E, Meehl JB, Voeltz GK. Dev Cell. 2022 Jun 6;57(11):1369-1382.e6. doi: 10.1016/j.devcel.2022.05.002. Epub 2022 May 23. 10.1016/j.devcel.2022.05.002 PubMed 35609616
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