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Addgene

σ3-mCherry
(Plasmid #186580)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186580 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 5272
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP3S1
  • Alt name
    AP-3 complex subunit sigma-1
  • Alt name
    CLAPS3
  • Species
    H. sapiens (human)
  • Entrez Gene
    AP3S1 (a.k.a. CLAPS3, Sigma3A)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer 5CMV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    σ3-mCherry was a gift from Stephen Royle (Addgene plasmid # 186580 ; http://n2t.net/addgene:186580 ; RRID:Addgene_186580)

  • For your References section:

    Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation. Kuey C, Sittewelle M, Larocque G, Hernandez-Gonzalez M, Royle SJ. Elife. 2022 Jul 19;11. pii: 78929. doi: 10.7554/eLife.78929. 10.7554/eLife.78929 PubMed 35852853
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