Lamp1-mCherry-FRB
(Plasmid #186576)

• Purpose: Expression of mCherry-FRB at the outer surface of the lysosome using LAMP1
• Depositing Lab: Stephen Royle
• Publication: Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi: 10.7554/eLife.78929.

Backbone

• Vector backbone: pMito
• Total vector size (bp): 6282
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LAMP1
• Alt name: Lysosome-associated membrane glycoprotein 1
• Species: H. sapiens (human)
• GenBank ID: NM_005561.4
• Entrez Gene: LAMP1 (a.k.a. CD107a, LAMPA, LGP120)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry-FRB (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pCMV

Resource Information

• Supplemental Documents:
  • Lamp1-mCherry-FRB_v2.gb
• A portion of this plasmid was derived from a plasmid made by: Addgene # 34831

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry