Skip to main content
Addgene

FRB-mCherry-Giantin
(Plasmid #186575)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186575 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMito
  • Total vector size (bp) 5370
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Giantin
  • Species
    H. sapiens (human)
  • GenBank ID
  • Entrez Gene
    GOLGB1 (a.k.a. GCP, GCP372, GOLIM1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FRB-mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pCMV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRB-mCherry-Giantin was a gift from Stephen Royle (Addgene plasmid # 186575 ; http://n2t.net/addgene:186575 ; RRID:Addgene_186575)

  • For your References section:

    Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation. Kuey C, Sittewelle M, Larocque G, Hernandez-Gonzalez M, Royle SJ. Elife. 2022 Jul 19;11. pii: 78929. doi: 10.7554/eLife.78929. 10.7554/eLife.78929 PubMed 35852853
Related items:
Commonly requested with: