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Addgene

FRB-mCherry-Sec61β
(Plasmid #186574)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186574 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMito
  • Total vector size (bp) 5296
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEC61B
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_006808.3
  • Entrez Gene
    SEC61B
  • Promoter CMV
  • Tag / Fusion Protein
    • FRB-mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pCMV
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene #15108

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRB-mCherry-Sec61β was a gift from Stephen Royle (Addgene plasmid # 186574 ; http://n2t.net/addgene:186574 ; RRID:Addgene_186574)

  • For your References section:

    Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation. Kuey C, Sittewelle M, Larocque G, Hernandez-Gonzalez M, Royle SJ. Elife. 2022 Jul 19;11. pii: 78929. doi: 10.7554/eLife.78929. 10.7554/eLife.78929 PubMed 35852853
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