pMito-dCherry-FRB
(Plasmid #186573)

• Purpose: Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells
• Depositing Lab: Stephen Royle
• Publication: Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi: 10.7554/eLife.78929.

Backbone

• Vector backbone: pMito
• Total vector size (bp): 5132
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dark MitoTrap
• Mutation: Mutation of "K70N" to render mCherry non-fluorescent
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Age I (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: CMV Forward

Resource Information

• Supplemental Documents:
  • pMito-dCherry-FRB.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mutation of pMito-mCherry-FRB (Addgene 59352) to introduce "K70N" mutation