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Pet15b-Internalization and Endosomal Escape
(Plasmid #186551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Pet15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5708
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    10R-8His-ETA-EGFP
  • Alt name
    Exotoxin A
  • Species
    Bacteria
  • Insert Size (bp)
    1274
  • Promoter T7
  • Tags / Fusion Proteins
    • 8xHis (N terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pet15b-Internalization and Endosomal Escape was a gift from Jean Gariepy (Addgene plasmid # 186551 ; http://n2t.net/addgene:186551 ; RRID:Addgene_186551)

  • For your References section:

    The Pseudomonas aeruginosa exotoxin A translocation domain facilitates the routing of CPP-protein cargos to the cytosol of eukaryotic cells. Mohammed AF, Abdul-Wahid A, Huang EH, Bolewska-Pedyczak E, Cydzik M, Broad AE, Gariepy J. J Control Release. 2012 Nov 28;164(1):58-64. doi: 10.1016/j.jconrel.2012.10.006. Epub 2012 Oct 14. 10.1016/j.jconrel.2012.10.006 PubMed 23075769
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