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Addgene

HA-GbL
(Plasmid #1865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK-5
  • Backbone manufacturer
    BD Pharmingen
  • Backbone size w/o insert (bp) 4754
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GbL
  • Alt name
    G beta L
  • Alt name
    G protein beta subunit-like
  • Species
    H. sapiens (human)
  • Entrez Gene
    MLST8 (a.k.a. GBL, GbetaL, LST8, POP3, WAT1)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-GbL was a gift from David Sabatini (Addgene plasmid # 1865 ; http://n2t.net/addgene:1865 ; RRID:Addgene_1865)

  • For your References section:

    Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. Curr Biol 2004 Jul 27;14(14):1296-302. 10.1016/j.cub.2004.06.054 PubMed 15268862
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