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pQCXIP EGFP-ER [TM(SAC1)]
(Plasmid #186475)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186475 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6991
  • Total vector size (bp) 7957
  • Modifications to backbone
    Replacement of the puromycin resistance gene by a blasticidine resistance gene
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SAC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    204
  • Mutation
    C-terminal transmembrane region of the protein: 521-587
  • GenBank ID
    XM_011533500.3
  • Entrez Gene
    SACM1L (a.k.a. SAC1)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Tamas Balla

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Visualization and manipulation of plasma membrane-endoplasmic reticulum contact sites indicates the presence of additional molecular components within the STIM1-Orai1 Complex. Péter Várnai, Balázs Tóth, Dániel J Tóth, László Hunyady, Tamas Balla
J Biol Chem. 2007 Oct 5;282(40):29678-90. PMID: 17684017 DOI: 10.1074/jbc.M704339200

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIP EGFP-ER [TM(SAC1)] was a gift from Catherine Tomasetto (Addgene plasmid # 186475 ; http://n2t.net/addgene:186475 ; RRID:Addgene_186475)

  • For your References section:

    STARD3 or STARD3NL and VAP form a novel molecular tether between late endosomes and the ER. Alpy F, Rousseau A, Schwab Y, Legueux F, Stoll I, Wendling C, Spiegelhalter C, Kessler P, Mathelin C, Rio MC, Levine TP, Tomasetto C. J Cell Sci. 2013 Dec 1;126(Pt 23):5500-12. doi: 10.1242/jcs.139295. Epub 2013 Oct 8. 10.1242/jcs.139295 PubMed 24105263
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