pET15b MOSPD2 [315-445]
(Plasmid
#186474)
-
PurposeExpression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 186474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET-15b
-
Backbone manufacturerNovagen (EMD Millipore)
- Backbone size w/o insert (bp) 5708
- Total vector size (bp) 6099
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMOSPD2 motile sperm domain containing 2
-
SpeciesH. sapiens (human)
-
Insert Size (bp)393
-
MutationMSP domain [315-445]
-
GenBank IDNM_152581
-
Entrez GeneMOSPD2
-
Tag
/ Fusion Protein
- 6HIS (N terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET15b MOSPD2 [315-445] was a gift from Catherine Tomasetto (Addgene plasmid # 186474 ; http://n2t.net/addgene:186474 ; RRID:Addgene_186474) -
For your References section:
FFAT motif phosphorylation controls formation and lipid transfer function of inter-organelle contacts. Di Mattia T, Martinet A, Ikhlef S, McEwen AG, Nomine Y, Wendling C, Poussin-Courmontagne P, Voilquin L, Eberling P, Ruffenach F, Cavarelli J, Slee J, Levine TP, Drin G, Tomasetto C, Alpy F. EMBO J. 2020 Dec 1;39(23):e104369. doi: 10.15252/embj.2019104369. Epub 2020 Oct 30. 10.15252/embj.2019104369 PubMed 33124732