pQCXIP GFP-MOSPD2 W201E
(Plasmid
#186471)
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PurposeExpression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186471 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQCXIP
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 7181
- Total vector size (bp) 9459
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMOSPD2 motile sperm domain containing 2
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SpeciesH. sapiens (human)
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Insert Size (bp)1554
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MutationW201E (no more binding to lipid droplets)
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GenBank IDNM_152581
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Entrez GeneMOSPD2
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQCXIP GFP-MOSPD2 W201E was a gift from Catherine Tomasetto (Addgene plasmid # 186471 ; http://n2t.net/addgene:186471 ; RRID:Addgene_186471) -
For your References section:
MOSPD2 is an endoplasmic reticulum-lipid droplet tether functioning in LD homeostasis. Zouiouich M, Di Mattia T, Martinet A, Eichler J, Wendling C, Tomishige N, Grandgirard E, Fuggetta N, Fromental-Ramain C, Mizzon G, Dumesnil C, Carpentier M, Reina-San-Martin B, Mathelin C, Schwab Y, Thiam AR, Kobayashi T, Drin G, Tomasetto C, Alpy F. J Cell Biol. 2022 Jun 6;221(6). pii: 213116. doi: 10.1083/jcb.202110044. Epub 2022 Apr 7. 10.1083/jcb.202110044 PubMed 35389430