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Addgene

pQCXIP GFP-MOSPD2 W201E
(Plasmid #186471)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186471 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pQCXIP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7181
  • Total vector size (bp) 9459
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MOSPD2 motile sperm domain containing 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1554
  • Mutation
    W201E (no more binding to lipid droplets)
  • GenBank ID
    NM_152581
  • Entrez Gene
    MOSPD2
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQCXIP GFP-MOSPD2 W201E was a gift from Catherine Tomasetto (Addgene plasmid # 186471 ; http://n2t.net/addgene:186471 ; RRID:Addgene_186471)
  • For your References section:

    MOSPD2 is an endoplasmic reticulum-lipid droplet tether functioning in LD homeostasis. Zouiouich M, Di Mattia T, Martinet A, Eichler J, Wendling C, Tomishige N, Grandgirard E, Fuggetta N, Fromental-Ramain C, Mizzon G, Dumesnil C, Carpentier M, Reina-San-Martin B, Mathelin C, Schwab Y, Thiam AR, Kobayashi T, Drin G, Tomasetto C, Alpy F. J Cell Biol. 2022 Jun 6;221(6). pii: 213116. doi: 10.1083/jcb.202110044. Epub 2022 Apr 7. 10.1083/jcb.202110044 PubMed 35389430