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Addgene

pET15b MOSPD2 RD/LD [282-490]
(Plasmid #186470)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186470 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    Novagen (EMD Millipore)
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 6291
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MOSPD2 motile sperm domain containing 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    627
  • Mutation
    MSP domain [282-490]
  • GenBank ID
    NM_152581
  • Entrez Gene
    MOSPD2
  • Tag / Fusion Protein
    • 6HIS (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b MOSPD2 RD/LD [282-490] was a gift from Catherine Tomasetto (Addgene plasmid # 186470 ; http://n2t.net/addgene:186470 ; RRID:Addgene_186470)
  • For your References section:

    Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites. Di Mattia T, Wilhelm LP, Ikhlef S, Wendling C, Spehner D, Nomine Y, Giordano F, Mathelin C, Drin G, Tomasetto C, Alpy F. EMBO Rep. 2018 Jul;19(7). pii: embr.201745453. doi: 10.15252/embr.201745453. Epub 2018 Jun 1. 10.15252/embr.201745453 PubMed 29858488