pET15b MOSPD2 [282-490]
(Plasmid
#186469)
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PurposeExpression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186469 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-15b
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Backbone manufacturerNovagen (EMD Millipore)
- Backbone size w/o insert (bp) 5708
- Total vector size (bp) 6291
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMOSPD2 motile sperm domain containing 2
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SpeciesH. sapiens (human)
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Insert Size (bp)627
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MutationMSP domain [282-490]
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GenBank IDNM_152581
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Entrez GeneMOSPD2
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Tag
/ Fusion Protein
- 6HIS (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b MOSPD2 [282-490] was a gift from Catherine Tomasetto (Addgene plasmid # 186469 ; http://n2t.net/addgene:186469 ; RRID:Addgene_186469) -
For your References section:
Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites. Di Mattia T, Wilhelm LP, Ikhlef S, Wendling C, Spehner D, Nomine Y, Giordano F, Mathelin C, Drin G, Tomasetto C, Alpy F. EMBO Rep. 2018 Jul;19(7). pii: embr.201745453. doi: 10.15252/embr.201745453. Epub 2018 Jun 1. 10.15252/embr.201745453 PubMed 29858488