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Nuc-meCpG
(Plasmid #186464)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186464 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV
  • Total vector size (bp) 5703
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dimeric MBD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    516
  • Entrez Gene
    MBD1 (a.k.a. CXXC3, PCM1, RFT)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMVf
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Nuc-meCpG was a gift from Chongli Yuan (Addgene plasmid # 186464 ; http://n2t.net/addgene:186464 ; RRID:Addgene_186464)

  • For your References section:

    Live-Cell Probe for In Situ Single-Cell Monitoring of Mitochondrial DNA Methylation. Zhao H, Ma D, Xie J, Sanchez O, Huang F, Yuan C. ACS Sens. 2021 Oct 22;6(10):3575-3586. doi: 10.1021/acssensors.1c00731. Epub 2021 Sep 29. 10.1021/acssensors.1c00731 PubMed 34586782
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