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pCS2-mCherry-Cenexin-S796A
(Plasmid #186434)

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Item Catalog # Description Quantity Price (USD)
Plasmid 186434 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4091
  • Total vector size (bp) 7935
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cenexin
  • Alt name
    ODF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3844
  • Mutation
    Changed Serine 796 to Alanine
  • GenBank ID
    NM_153435.1
  • Entrez Gene
    ODF2 (a.k.a. CT134, ODF2/1, ODF2/2, ODF84)
  • Promoter CMV IE94
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Kyung S Lee Lab of the National Cancer Institute and Stephen Doxsey Lab University of Massachusetts Medical School Program in Molecular Medicine

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Published in: Soung, N.-K., Kang, Y. H., Kim, K., Kamijo, K., Yoon, H., Seong, Y.-S., Kuo, Y.-L., Miki, T., Kim, S. R., Kuriyama, R., Giam, C.-Z., Ahn, C. H., & Lee, K. S. (2006). Requirement of hCenexin for Proper Mitotic Functions of Polo-Like Kinase 1 at the Centrosomes. Molecular and Cellular Biology, 26(22), 8316–8335. https://doi.org/10.1128/mcb.00671-06

Hung HF, Hehnly H, Doxsey S. The Mother Centriole Appendage Protein Cenexin Modulates Lumen Formation through Spindle Orientation. Curr Biol. 2016 Mar 21;26(6):793-801. doi: 10.1016/j.cub.2016.01.025. Epub 2016 Mar 3. PMID: 26948879; PMCID: PMC5027652.

Please visit https://www.biorxiv.org/content/10.1101/2022.01.09.475500v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-mCherry-Cenexin-S796A was a gift from Heidi Hehnly (Addgene plasmid # 186434 ; http://n2t.net/addgene:186434 ; RRID:Addgene_186434)

  • For your References section:

    Pericentriolar matrix (PCM) integrity relies on cenexin and polo-like kinase (PLK)1. Aljiboury A, Mujcic A, Curtis E, Cammerino T, Magny D, Lan Y, Bates M, Freshour J, Ahmed-Braimeh YH, Hehnly H. Mol Biol Cell. 2022 Aug 1;33(9):br14. doi: 10.1091/mbc.E22-01-0015. Epub 2022 May 24. 10.1091/mbc.E22-01-0015 PubMed 35609215
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