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Addgene

ZS007:eGFP-TM
(Plasmid #186429)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186429 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDisplay
  • Backbone size w/o insert (bp) 4586
  • Total vector size (bp) 6019
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    eGFP-TM
  • Species
    Synthetic
  • Insert Size (bp)
    1433
  • Promoter cmv

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gattatgctggggcccTGGTGAGCAAGGGCGAGG
  • 3′ sequencing primer tcgacctgcagccgcggCTTGTACAGCTCGTCCATGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZS007:eGFP-TM was a gift from Adam Cohen (Addgene plasmid # 186429 ; http://n2t.net/addgene:186429 ; RRID:Addgene_186429)

  • For your References section:

    Cell Membranes Resist Flow. Shi Z, Graber ZT, Baumgart T, Stone HA, Cohen AE. Cell. 2018 Dec 13;175(7):1769-1779.e13. doi: 10.1016/j.cell.2018.09.054. Epub 2018 Nov 1. 10.1016/j.cell.2018.09.054 PubMed 30392960
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