Skip to main content
Addgene

dCas9 fused to scFv (anti-spike)
(Plasmid #186421)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pdCas9-bacteria
  • Backbone manufacturer
    Addgene #44249 Stanley Qi
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-scFv fusion (anti-SARS-CoV-2 spike)
  • Species
    Synthetic
  • Insert Size (bp)
    4971
  • Tag / Fusion Protein
    • 6His

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Backbone derived from Addgene #44249 scFv fusion is B1-182.1 from Wang et al "Ultrapotent antibodies against diverse and highly transmissible SARS-CoV-2 variants" Science 2021

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dCas9 fused to scFv (anti-spike) was a gift from Stephen Elledge (Addgene plasmid # 186421 ; http://n2t.net/addgene:186421 ; RRID:Addgene_186421)

  • For your References section:

    CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profiling. Barber KW, Shrock E, Elledge SJ. Cell Rep Methods. 2022 Oct 17;2(10):100318. doi: 10.1016/j.crmeth.2022.100318. eCollection 2022 Oct 24. 10.1016/j.crmeth.2022.100318 PubMed 36313802
Related items: