scramble shRNA
(Plasmid #1864)

• Purpose: 3rd gen lentiviral negative control vector containing scrambled shRNA
• Depositing Lab: David Sabatini
• Publication: Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Backbone

• Vector backbone: pLKO.1
• Backbone manufacturer: Stewart SA, RNA 2003 Apr; 9(4):493-501.
• Backbone size w/o insert (bp): 7032
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Growth instructions: XL10-Gold Ultracompetent Cells from Stratagene. 37oC.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: scramble
• Insert Size (bp): 60

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• Reference:
  • Addgene's pLKO.1 protocol
• Articles Citing this Plasmid:
  • 567 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

shRNA for use as a negative control. Sequence of hairpin is:CCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGG. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454).

Please note that the 5' cloning site, AgeI, is typically destroyed during the shRNA cloning. Depending on the specific shRNA sequence, the site can occasionally be restored. AgeI is present in this plasmid.