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pENT-L3-a-element-L4
(Plasmid #186366)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186366 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR-221-P3-P5
  • Backbone manufacturer
    PMID: 17878951
  • Backbone size w/o insert (bp) 2550
  • Vector type
    Protein expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    a-element early Otx neural enhancer
  • Species
    C. intestinalis (ascidian)
  • Insert Size (bp)
    61
  • Promoter T7 promoter

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13forward: TGTAAAACGACGGCCAGT
  • 3′ sequencing primer M13rev: caggaaacagctatgac
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ciona a-element : https://www.aniseed.cnrs.fr/aniseed/gene/show_gene?unique_id=Cirobu.g00006940.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Ciona Otx a-element, drives expression in a6.5, b6.5 lineages from the late 32-cell stage.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENT-L3-a-element-L4 was a gift from Patrick Lemaire (Addgene plasmid # 186366 ; http://n2t.net/addgene:186366 ; RRID:Addgene_186366)

  • For your References section:

    A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos. Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero C, Mueller F, Lemaire P. PLoS One. 2007. 2(9):e916. 10.1371/journal.pone.0000916 PubMed 17878951
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