Skip to main content
Addgene

pENT-L3-pFOGl-L5
(Plasmid #186364)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186364 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDONR-221-P3-P5
  • Backbone manufacturer
    PMID: 17878951
  • Backbone size w/o insert (bp) 2550
  • Vector type
    Protein expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Friend Of Gata regulatory sequence
  • Species
    C. intestinalis (ascidian)
  • Insert Size (bp)
    1987
  • Promoter T7 promoter

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13forward: TGTAAAACGACGGCCAGT
  • 3′ sequencing primer M13rev: caggaaacagctatgac
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ciona pFOGl : https://www.aniseed.cnrs.fr/aniseed/gene/show_gene?unique_id=Cirobu.g00001764.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Ci-FOG cis-regulatory region drives expression in Ciona animal territories from the 16-cell stage.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENT-L3-pFOGl-L5 was a gift from Patrick Lemaire (Addgene plasmid # 186364 ; http://n2t.net/addgene:186364 ; RRID:Addgene_186364)

  • For your References section:

    A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos. Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero C, Mueller F, Lemaire P. PLoS One. 2007. 2(9):e916. 10.1371/journal.pone.0000916 PubMed 17878951
Related items: