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pENT-L1-Kozak-NLSlacZ-stop-L2
(Plasmid #186358)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186358 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PDONR-221 P1-P2
  • Backbone manufacturer
    PMID: 14736459
  • Backbone size w/o insert (bp) 2544
  • Vector type
    Expression of nuclear LacZ reporter

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lacZ
  • Species
    Synthetic; E. coli
  • Insert Size (bp)
    3213
  • Entrez Gene
    lacZ (a.k.a. b0344, ECK0341)
  • Tag / Fusion Protein
    • Nuclear localization signal (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13forward: TGTAAAACGACGGCCAGT
  • 3′ sequencing primer M13rev: caggaaacagctatgac
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    NLS-LacZ was a kind gift from R. Di Lauro.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENT-L1-Kozak-NLSlacZ-stop-L2 was a gift from Patrick Lemaire (Addgene plasmid # 186358 ; http://n2t.net/addgene:186358 ; RRID:Addgene_186358)

  • For your References section:

    A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos. Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero C, Mueller F, Lemaire P. PLoS One. 2007. 2(9):e916. 10.1371/journal.pone.0000916 PubMed 17878951
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