pENT-L1-Kozak-GAP43-ECFP-stop-L2
(Plasmid
#186356)
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PurposeEntry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequences
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186356 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePDONR-221 P1-P2
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Backbone manufacturerPMID: 14736459
- Backbone size w/o insert (bp) 2544
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Vector typeExpression of a fluorescent membrane marker
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGrowth-associated protein-43
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SpeciesM. musculus (mouse), Synthetic
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Insert Size (bp)821
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Entrez GeneGap43 (a.k.a. B-50, Basp2, GAP-43)
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Tag
/ Fusion Protein
- ECFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13forward: TGTAAAACGACGGCCAGT
- 3′ sequencing primer M13rev: caggaaacagctatgac (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bythe GAP43-ECFP were kind gifts from Dr. F. Fagotto (CRBM-CNRS UMR5237, France).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Erroneously annotated in the article PMID 17878951 as pENT-L1-Kozak-GAP43-GFP-stop-L2
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENT-L1-Kozak-GAP43-ECFP-stop-L2 was a gift from Patrick Lemaire (Addgene plasmid # 186356 ; http://n2t.net/addgene:186356 ; RRID:Addgene_186356) -
For your References section:
A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos. Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero C, Mueller F, Lemaire P. PLoS One. 2007. 2(9):e916. 10.1371/journal.pone.0000916 PubMed 17878951