pENT-L1-Kozak-EGFP-GPI-stop-L2
(Plasmid #186355)

• Purpose: Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences
• Depositing Lab: Patrick Lemaire
• Publication: Roure et al PLoS One. 2007. 2(9):e916.

Backbone

• Vector backbone: PDONR-221 P1-P2
• Backbone manufacturer: PMID: 14736459
• Backbone size w/o insert (bp): 2544
• Vector type: Expression of a fluorescent membrane marker

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: signal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 936
• Entrez Gene: CD59 (a.k.a. 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20)
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13forward: TGTAAAACGACGGCCAGT
• 3′ sequencing primer: M13rev: caggaaacagctatgac

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry