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Addgene

pENT L1-Kozak-ERK-KTR-L2
(Plasmid #186353)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186353 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PDONR-221 P1-P2
  • Backbone manufacturer
    PMID: 14736459
  • Backbone size w/o insert (bp) 2544
  • Vector type
    Protein expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERK Kinase Translocation Reporter
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    261
  • Entrez Gene
    MAPK1 (a.k.a. ERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13forward: TGTAAAACGACGGCCAGT
  • 3′ sequencing primer M13rev: caggaaacagctatgac
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENT L1-Kozak-ERK-KTR-L2 was a gift from Patrick Lemaire (Addgene plasmid # 186353 ; http://n2t.net/addgene:186353 ; RRID:Addgene_186353)
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