pET24a_SMOX
(Plasmid
#186345)
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PurposeC-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 186345 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepet24a
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Backbone manufacturerGenscript
- Backbone size w/o insert (bp) 5255
- Total vector size (bp) 6920
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSpermine oxidase
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Alt nameSMOX
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SpeciesH. sapiens (human)
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Insert Size (bp)1665
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Entrez GeneSMOX (a.k.a. C20orf16, PAO, PAO-1, PAO1, PAOH, PAOH1, SMO)
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Tag
/ Fusion Protein
- His tag (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET24a_SMOX was a gift from Sandra Gabelli (Addgene plasmid # 186345 ; http://n2t.net/addgene:186345 ; RRID:Addgene_186345) -
For your References section:
Identification of a Novel Substrate-Derived Spermine Oxidase Inhibitor. Dunston TT, Khomutov MA, Gabelli SB, Stewart TM, Foley JR, Kochetkov SN, Khomutov AR, Casero RA Jr. Acta Naturae. 2020 Jul-Sep;12(3):140-144. doi: 10.32607/actanaturae.10992. 10.32607/actanaturae.10992 PubMed 33173604