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Purpose(Empty Backbone) Shuttle vector, pWVO1 origin of replication (from L. lactis sbsp. cremoris Wg2). Replicates in both Gram-Positive and Gram-Negative bacteria. Chloramphenicol- and Erythromycin resistance marker.
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186342 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepWV01
- Backbone size (bp) 4378
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Erythromycin, 25 & 200 μg/mL
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Growth Temperature30°C
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Growth Strain(s)E.coli BHB2600
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Growth instructionsThis plasmid can be propagated in a broad host range, including Lactococcus lactis, Escherichia coli, Bacillus subtilis and several Lactobacillus strains.
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Copy numberHigh Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGK12 was a gift from Jan Kok & Gerard Venema (Addgene plasmid # 186342) -
For your References section:
Construction of plasmid cloning vectors for lactic streptococci which also replicate in Bacillus subtilis and Escherichia coli. Kok J, van der Vossen JM, Venema G. Appl Environ Microbiol. 1984 Oct;48(4):726-31. doi: 10.1128/aem.48.4.726-731.1984. 10.1128/aem.48.4.726-731.1984 PubMed 6095756
Map uploaded by the depositor.
