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pClgn1.1-Trim41-PA-1D4
(Plasmid #186339)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186339 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pClgn1.1
  • Backbone manufacturer
    Masahito Ikawa
  • Backbone size w/o insert (bp) 3793
  • Total vector size (bp) 5774
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Trim41
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1989
  • Entrez Gene
    Trim41 (a.k.a. AW552703, BC020156, R75223, RINCK)
  • Promoter pClgn1.1
  • Tags / Fusion Proteins
    • PA (C terminal on insert)
    • 1D4 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 5'-TTGAGCGGGCCGCTTGCGCACTGG-3'
  • 3′ sequencing primer 5'-GCCACACCAGCCACCACCTTCTG-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClgn1.1-Trim41-PA-1D4 was a gift from Masahito Ikawa (Addgene plasmid # 186339 ; http://n2t.net/addgene:186339 ; RRID:Addgene_186339)

  • For your References section:

    Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice. Oura S, Hino T, Satoh T, Noda T, Koyano T, Isotani A, Matsuyama M, Akira S, Ishiguro KI, Ikawa M. PLoS Genet. 2022 Jun 1;18(6):e1010241. doi: 10.1371/journal.pgen.1010241. 10.1371/journal.pgen.1010241 PubMed 35648791
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